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Thioglycolate Medium, Fluid (Fluid Thioglycolate Medium) (FTG) (BAM M146)

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]

Thioglycolate Medium, Fluid

(Fluid Thioglycolate Medium)

(FTG)

(BAM M146)

山東龍8娛樂官方網站生物工程有限公司培養基配方 //gongningdiu1119.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Glucose ...............................................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................2.5g

Agar ..................................................................0.75g

L-cystine ..............................................................0.5g

Sodium thioglycolate ..........................................0.5g

Resazurin solution........................................... 1.0mL

pH 7.1 ± 0.2 at 25°C

Resazurin Solution:

Composition per 10.0mL:

Na-resazurin...................................................10.0mg

Preparation of Resazurin Solution: Add Na

resazurin to 10.0mL of distilled/deionized water. Mix

thoroughly. Prepare freshly.

Preparation of Medium: Add components, ex

cept sodium thioglycolate and resazurin solution, to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Add 0.5g sodium thioglycolate. Mix thoroughly. Ad

just pH to 7.1. Add 1.0mL resazurin solution. Mix

thoroughly. Distribute into tubes or flasks. Autoclave

for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of both aerobic and anaero

bic organisms in the performance of sterility tests.

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