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培養基配方
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Lactobacilli MRS Broth with Cysteine
[所屬分類:培養基配方] [發布時間:2021-4-26] [發布人:李佳頤] [閱讀次數:] [返回]
Lactobacilli MRS Broth with Cysteine
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine.....................................................................................2.0g
Ammonium citrate........................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Tween™ 80...................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·5H 2 O..............................................................................0.05g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation and maintenance of Lactobacillus species,
including L. ruminis and L. vitulinus.
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Composition per liter:
Glucose.......................................................................................20.0gBeef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine.....................................................................................2.0g
Ammonium citrate........................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Tween™ 80...................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·5H 2 O..............................................................................0.05g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation and maintenance of Lactobacillus species,
including L. ruminis and L. vitulinus.