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Thiamine Assay Medium

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]

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Composition per liter:

Glucose .............................................................40.0g

Peptone .............................................................22.0g

Sodium acetate..................................................15.0g

Vitamin assay casamino acids ............................5.0g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.4g

Adenine sulfate .................................................0.02g

FeSO4·7H2O......................................................0.02g

Guanine·HCl .....................................................0.02g

MnSO4·5H2O....................................................0.02g

NaCl..................................................................0.02g

Uracil ................................................................0.02g

L-Cystine ..........................................................0.2mg

p-Aminobenzoic acid.......................................0.2mg

Calcium pantothenate ......................................0.2mg

Niacin...............................................................0.2mg

Pyridoxine·HCl ................................................0.2mg

Riboflavin ........................................................0.2mg

Folic acid ..........................................................0.5μg

Biotin ................................................................0.8μg

pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes or

flasks in 5.0mL volumes while swirling the flask to

disperse the precipitate. Add standard solutions or

test solutions and bring volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine

using Lactobacillus fermentum as the test organism.

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