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Thiamine Assay Medium LV

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]

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Composition per liter:

Glucose .............................................................20.0g

Pancreatic digest of casein................................20.0g

K2HPO4.............................................................10.0g

NaCl..................................................................10.0g

Sodium citrate...................................................10.0g

Yeast extract, thiamine-free ..............................10.0g

Sorbitan monooleate complex ............................2.0g

MgSO4·7H2O ......................................................1.6g

MnCl2·4H2O .....................................................0.28g

FeSO4·7H2O......................................................0.08g

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes or

flasks in 5.0mL volumes while swirling the flask to

disperse the precipitate. Add standard solutions or

test solutions and bring volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine

using Lactobacillus viridescens as the test organism.

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