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Thioglycolate Gelatin Medium

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Gelatin Medium

山東龍8娛樂官方網站生物工程有限公司 培養基配方 //gongningdiu1119.com

Composition per liter:

Gelatin...............................................................50.0g
Pancreatic digest of casein................................15.0g
Yeast extract........................................................5.0g
NaCl....................................................................2.5g
Glucose ...............................................................2.0g
Agar ..................................................................0.75g
L-Cystine ...........................................................0.25g
Na2SO3................................................................0.1g
Thioglycollic acid ........................................... 0.3mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to 50°C. Let
stand 5 min. Gently heat and bring to boiling. Distrib
ute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the determination of gelatin liquefaction by
aerobes, microaerophiles, and anaerobes without
special incubation.
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