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Thioglycolate Medium (DSMZ Medium 530)

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Medium
(DSMZ Medium 530)

山東龍8娛樂官方網站生物工程有限公司 培養基配方 //gongningdiu1119.com

Composition per liter:

Agar ..................................................................15.0g
Peptone from casein............................................5.0g
Meat extract ........................................................3.0g
Na-thioglycolate solution............................ 100.0mL
pH 5.5 ± 0.2 at 25°C
Thioglycolate Solution :
Composition per 100mL:
Na-Thioglycolate ................................................0.5g
Preparation of Thioglycolate Solution: Add
thioglycolate to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°C.
Preparation of Medium: Add components, ex
cept thioglycolate solution to distilled/deionized wa
ter and bring volume to 900.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Asepti
cally add 100.0mL warm thioglycolate solution. Ad
just pH to 5.5. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation and maintenance of various
anaerobic bacteria.
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