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Thioglycolate Medium, Brewer

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Medium, Brewer

山東龍8娛樂官方網站生物工程有限公司 培養基配方 //gongningdiu1119.com

Composition per liter:

Glucose ...............................................................5.0g
Peptone................................................................5.0g
NaCl....................................................................5.0g
Yeast extract........................................................2.0g
Sodium thioglycolate ..........................................1.1g
Agar ....................................................................1.0g
Beef extract.........................................................1.0g
Methylene Blue................................................2.0mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For determination of the sterility of solutions
containing mercurial preservatives.
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