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Thioglycolate Medium, Brewer Modified

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]

Thioglycolate Medium, Brewer

Modified

山東龍8娛樂官方網站生物工程有限公司培養基配方 //gongningdiu1119.com

Composition per liter:

Pancreatic digest of casein................................17.5g

Glucose .............................................................10.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................2.5g

K2HPO4...............................................................2.0g

Sodium thioglycolate ..........................................1.0g

Agar ....................................................................0.5g

Methylene Blue...............................................0.002g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks, filling them

half full. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of obligate anaerobes, mi

croaerophiles, and facultative organisms.

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