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Thioglycolate Medium with 20% Bile (THIO + Bile Medium)

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Medium with 20% Bile
(THIO + Bile Medium)

山東龍8娛樂官方網站生物工程有限公司 培養基配方 //gongningdiu1119.com

Composition per liter:

Oxgall ...............................................................20.0g
Thioglycolate medium without indicator........... 1.0L
Hemin solution................................................ 0.5mL
Vitamin K1 solution ........................................ 0.1mL
pH 7.0 ± 0.2 at 25°C
Thioglycolate Medium without Indicator:
Composition per liter:
Pancreatic digest of casein................................17.0g
Glucose ...............................................................6.0g
Papaic digest of soybean meal............................3.0g
NaCl....................................................................2.5g
Agar ....................................................................0.7g
Sodium thioglycolate..........................................0.5g
L-Cystine ...........................................................0.25g
Na2SO3................................................................0.1g
Preparation of Thioglycolate Medium with
out Indicator: Add components to distilled/deion
ized water and bring volume to 1.0L. Mix thoroughly.
Vitamin K1 Solution:
Composition per 100.0mL:
Vitamin K1 ..........................................................1.0g
Preparation of Vitamin K1 Solution: Add vita
min K1 to 99.0mL of absolute ethanol. Mix thorough
ly.
Hemin Solution:
Composition per 100.0mL:
Hemin .................................................................1.0g
NaOH (1N solution)...................................... 20.0mL
Preparation of Hemin Solution: Add hemin to
20.0mL of 1N NaOH solution. Mix thoroughly.
Bring volume to 100.0mL with distilled/deionized
water.
Preparation of Medium: Add 0.5mL of hemin
solution, 0.1mL of vitamin K1 solution, and oxgall to
1.0L of thioglycolate medium without indicator. Mix
thoroughly. Distribute into screw-capped tubes or
flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool tubes or flasks under 85% N2 + 10% H2
+ 5% CO2. Tighten caps.
Use: For the isolation, cultivation, and identification of
a variety of obligate anaerobic bile-tolerant bacteria.
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