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培養基配方
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Thioglycolate Medium without Glucose
[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Medium without Glucose
山東龍8娛樂官方網站生物工程有限公司 培養基配方 //gongningdiu1119.com
Composition per liter:
Pancreatic digest of casein................................15.0g
Yeast extract........................................................5.0g
NaCl....................................................................2.5g
Agar ..................................................................0.75g
L-Cystine ...........................................................0.25g
Methylene Blue................................................2.0mg
Thioglycollic acid ........................................... 0.3mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. If medium becomes oxi
dized before use (Methylene Blue turns blue), heat in
a boiling water bath to expel absorbed O2. Cool to
25°C.
Use: For the cultivation of anaerobic, microaero
philic, and aerobic microorganisms. For use in steril
ity testing of a variety of specimens.